Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress

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Suppression subtractive hybridization.

Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...

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transcriptome of pigeonpea roots under water deficit analyzed by suppression subtractive hybridization

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A Mathematical Model for Suppression Subtractive Hybridization

Suppression subtractive hybridization (SSH) is frequently used to unearth differentially expressed genes on a whole-genome scale. Its versatility is based on combining cDNA library subtraction and normalization, which allows the isolation of sequences of varying degrees of abundance and differential expression. SSH is a complex process with many adjustable parameters that affect the outcome of ...

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ژورنال

عنوان ژورنال: Journal of Integrative Agriculture

سال: 2014

ISSN: 2095-3119

DOI: 10.1016/s2095-3119(13)60578-x